Effect of autologous platelet-rich plasma on human sperm quality during cryopreservation

As a source of growth factors, platelet-rich plasma (PRP) has been widely used in the repair various injuries due to its cytoprotective properties regenerative medicine. The objective present study was investigate effect autologous PRP supplementation on quality frozen-thawed human sperm. Twelve normozoospermic semen samples were collected, and each sample divided into 4 aliquots added with different proportions (0%, 2%, 5%, 10%) separately, followed by cryopreservation. Sperm motility, viability, membrane integrity, DNA fragmentation index (DFI), reactive oxygen species (ROS) levels mitochondrial potential measured analyzed. results showed that addition 5% improved sperm progressive motility (30.3 ± 2.7 VS. 28.1 2.6), viability (65.5 4.2 59.6 3.9), integrity (52.4 3.6 49.2 3.4) after cryopreservation (P < 0.05). Slight attenuation ROS generation, restoration reduction fragments also observed post-thawed supplemented PRP, but there no significant > In conclusion, partial protective spermatozoa, combined application other high-efficiency cryoprotectant is worthy further study.